EdenteV2, Main, Exploration, bibRecord, 00A426

Protease repertoires of cells adherent to membranes recovered after guided tissue regeneration

Identifieur interne : 00A426 ( Main/Exploration ); précédent : 00A425; suivant : 00A427

Protease repertoires of cells adherent to membranes recovered after guided tissue regeneration

Auteurs : R. C. Wakabayashi [États-Unis] ; F. Wong [États-Unis] ; D. W. Richards [États-Unis] ; P. W. Johnson [États-Unis]

Source :

Journal of periodontal research [ 0022-3484 ] ; 1996.

RBID : Pascal:96-0330606

Descripteurs français

Pascal (Inist)
- Parodontopathie, Régénération, Os alvéolaire, Guide, Méthode blotting, Proteinases, Cicatrisation, Minéralisation, Etude expérimentale, Physiopathologie, Culture cellulaire, In vitro, Homme, Etude comparative, Zymogramme.
Wicri :
- topic : Guide, Homme.

English descriptors

KwdEn :
- Alveolar bone, Blotting assay, Cell culture, Cicatrization, Comparative study, Experimental study, Guide, Human, In vitro, Mineralization, Pathophysiology, Periodontal disease, Proteinases, Regeneration.

Abstract

Guided tissue regeneration is a clinical procedure used to restore mineralized tissue that has been lost to periodontal disease or after tooth extraction. The procedure makes use of Gore-tex® membranes or Gore-tex® augmentation membranes (GTAM) to prevent migration of keratinocytes and gingival fibroblasts into healing wounds. To begin to characterize the regenerative cells associated with these membranes, human cells have been rescued from membranes retrieved after bone-inductive procedures. Cell lines were established from tissue adherent to Gore-tex® membranes used to regenerate bone around periodontally compromised teeth, and from tissue adherent to GTAM used in edentulous ridge augmentation procedures or in conjunction with implant placement. Cell lines were screened for mineralized nodule formation in vitro prior to their subsequent analysis. All but one of the lines selected for this study formed mineralized nodules in vitro with cells from GTAM tending to form nodules more quickly than cells from Gore-tex®. Zymograms and Western blots were used to compare protease profiles of these cells with those of human gingival fibroblasts, keratinocytes and periodontal ligament (PDL) cells. All cell types except for keratinocytes produced a 72 kD protease. In contrast, keratinocytes were the only cells that produced 92 kD gelatinase. In some cell lines, notably those removed from patients after short periods of regeneration, collagenase was the major protease detected on gelatin substrate gels. Some of these cell lines also produced additional proteases including a low molecular weight protease (30 kD) not seen in gingival fibroblasts, PDL cells or keratinocytes.

Affiliations:

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Le document en format XML

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<term>Blotting assay</term>
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<term>Cicatrization</term>
<term>Comparative study</term>
<term>Experimental study</term>
<term>Guide</term>
<term>Human</term>
<term>In vitro</term>
<term>Mineralization</term>
<term>Pathophysiology</term>
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<term>Régénération</term>
<term>Os alvéolaire</term>
<term>Guide</term>
<term>Méthode blotting</term>
<term>Proteinases</term>
<term>Cicatrisation</term>
<term>Minéralisation</term>
<term>Etude expérimentale</term>
<term>Physiopathologie</term>
<term>Culture cellulaire</term>
<term>In vitro</term>
<term>Homme</term>
<term>Etude comparative</term>
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<front><div type="abstract" xml:lang="en">Guided tissue regeneration is a clinical procedure used to restore mineralized tissue that has been lost to periodontal disease or after tooth extraction. The procedure makes use of Gore-tex® membranes or Gore-tex® augmentation membranes (GTAM) to prevent migration of keratinocytes and gingival fibroblasts into healing wounds. To begin to characterize the regenerative cells associated with these membranes, human cells have been rescued from membranes retrieved after bone-inductive procedures. Cell lines were established from tissue adherent to Gore-tex® membranes used to regenerate bone around periodontally compromised teeth, and from tissue adherent to GTAM used in edentulous ridge augmentation procedures or in conjunction with implant placement. Cell lines were screened for mineralized nodule formation in vitro prior to their subsequent analysis. All but one of the lines selected for this study formed mineralized nodules in vitro with cells from GTAM tending to form nodules more quickly than cells from Gore-tex®. Zymograms and Western blots were used to compare protease profiles of these cells with those of human gingival fibroblasts, keratinocytes and periodontal ligament (PDL) cells. All cell types except for keratinocytes produced a 72 kD protease. In contrast, keratinocytes were the only cells that produced 92 kD gelatinase. In some cell lines, notably those removed from patients after short periods of regeneration, collagenase was the major protease detected on gelatin substrate gels. Some of these cell lines also produced additional proteases including a low molecular weight protease (30 kD) not seen in gingival fibroblasts, PDL cells or keratinocytes.</div>
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Protease repertoires of cells adherent to membranes recovered after guided tissue regeneration

Protease repertoires of cells adherent to membranes recovered after guided tissue regeneration

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri